The assessment of the seismic behavior of high-speed railway bridge is important because of the strategic nature of these structures. Estimating and predicting damage to the bridge caused by earthquakes of varying intensity can provide useful information, which will be very useful in managing the potential crisis. One of the most useful tools for estimating the damage caused by earthquakes to bridges is the improvement of fragility curves related to it. Studies in the production of fragility curves on the bridges of a high-speed rail are very limited. In this research, the fragility curve is plotted for two high-speed railway bridges with different column elevations. Due to the difference in the height of these bridges, a comparison of the performance of these structures is also shown. The model of the high-speed rail system was created for each model separately in the simulation software. Soil and structure interaction is modeled as a spring and its effects are considered. Nonlinear models are also used to model concrete and steel materials. Then, an incremental dynamic analysis is performed under the influence of different ground motions and the required data are extracted. Subsequently, appropriate limit states ​​were selected using these data, and finally, the fragility curve was plotted for the performance levels. The results showed that, with increasing column height, the damage index increased and also, for a constant probability, the longer column will be achieve a lower spectral acceleration to reach a performance level.