The main objective of this study is providing comparative results of cable stayed bridges under non-uniform and uniform excitations. For this purpose, a cable stayed bridges was modeled in SAP2000 software and imposed to two different uniform and non-uniform displacement time series. Two different soil types were considered for generating two different load cases. Twenty displacement time series, generated using spectral representation method, were considered in each load case for obtaining the bridge responses. Bridge responses were obtained in terms of deck and cable’s internal forces. In this paper, for the first time, the cable axial force variation under non-uniform excitation was investigated. Results of this research indicate that depending on the soil type beneath each support, the response of bridge may vary significantly. Deck stiffness is an important parameter for describing the amount of deck moment or cable’s axial force amplification. Deck moment, cables axial force are amplified up to 1.6, 1.2 respectively in the worst load case.