In construction industry, Concrete is a majorly used construction material and cement is the only manufactured material and other materials like coarse aggregate, fine aggregate and water are raw resources. In manufacturing of cement 900 kg of carbon dioxide is discharged per 1 T of Ordinary Portland Cement (OPC) production, which results in global warming. Several methods have been proposed to reduce the amount of cement in the manufacture of concrete. To minimize the adverse effects of carbon dioxide and to conserve energy, in this study it is determined to use bacteria in C40 grade concrete and to intensify its properties of hardened concrete. The innards for concrete are examined. In this study the strength of structural elements where examined with partial replacement of cement by fly ash enriched with bacteria.The compressive strength and tensile strength of concrete and flexural strength of reinforced concrete were studied in different concrete mixtures. The results show well effects of using enriched fly ash with bacteria on the concretes strength.